Molecular Diagnosis of Newcastle Disease Virus by RT-RCR and Detection Fusion Gene Using Conventional PCR
نویسندگان
چکیده
منابع مشابه
Differentiation of Virulent and Non-Virulent Newcastle Disease Virus Isolates Using RT-PCR
Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...
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Infectious bronchitis (IB) and Newcastle disease (ND) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. In the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-RT-PCR. RNA was extracted...
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Aim: Newcastle disease is still one of the major threats for poultry industry allover the world. Therefore, attempt was made in this study to use the SYBR Green I real-time PCR with melting curves analysis as for detection and differentiation of NDV strains in suspected infected birds. Materials and Methods: Two sets of primers were used to amplify matrix and fusion genes in samples collected f...
متن کاملRapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR.
A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted N...
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RT-PCR for the detection of Newcastle disease virus (NDV) in allantoic fluids of SPF embryonated eggs as well as in tissues of SPF chickens infected experimentally is described. The method proved to be specific as all tested NDVs were detected and no cross reaction with other RNA viruses was observed. Sensitivity of the method was established at 10ELD50/0.1 ml. To detect NDV in chicken tissues,...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical Negative Results
سال: 2022
ISSN: ['0976-9234', '2229-7723']
DOI: https://doi.org/10.47750/pnr.2022.13.s03.144